I agree, it's the best if OP don't move anything from the bitcoin that he already has and instead use the future money that's supposed to be for increasing what he's hodling to support that business. One thing I've noticed in that problem is that OP didn't mention if it's his business or not, he's just saying that he's supporting a business that's accepting a crypto, now unless I know if he's an investor to that business or he just wants to support it, I would be giving a different answer. If he's like a part of that business then maybe he needs to do some investing in it or something like that but if he's not then I guess what Cantsay said is what OP should follow or maybe just stick to investing.

I'm seeing quite a few replies that haven't even gone through reading the three lines of text in the original starting post, in a thread that is barely 3 pages long.

Although I understand the OP's concern about supporting a local business accepting Bitcoin, it really depends on your personal choice on how you manage your finances and how costly these visits to this business are. Are you buying coffee and merely spending $10 each time? If the answer is yes, you're stressing too much for a relatively small amount of bitcoin. Personally, I'd rather not spend Bitcoin when I can afford to pay with fiat because I'm also a long-term holder and haven't touched my coins for over 2.5 years. I don't see the reason to spend something that is now considered an asset and may double in value within the next few years. If you're keen on using Bitcoin as a payment in this particular business, I'd simply buy back any coins spent there or have a small balance of $50–$100 to be used only for this purpose.

Being in a dilemma situation can be very confusing. I have experienced it when I had more funds in my business and thought whether I should open a new branch or I should invest it in some assets that I had also entered before such as Real Estate and Bitcoin. But then I started to reflect further on the impact and consequences of each step I would take. And look at the risks and potential that I can take in the steps I will choose. So finally I found the answer that I prefer the convenience of using the extra money I have to add to my investments. Because I considered the geoeconomic situation at that time.

And I think in your case you just need to reflect more on yourself. look deeper into yourself and determine the option that you are most comfortable with if you choose that. Remember comfort comes from the deepest heart. Meanwhile, ambition and greed come from emotions that sometimes forget the risks. So like the others said. Follow your heart. And determine what is most profitable for you and you are comfortable with that choice.

Absolutely correct, in this life, its important to set principles and follow then strictly, you don't need to bend your rules to be a good person, thereby dysphonic your own plan to be successful. OP, I know its good to be charitable, but if I were you, I'll look for a way to acquire more btc to invest in the business. I should've told you to scrutinize the business and know for yourself if its ROI is encouraging, then hustle more to find finds to invest in it. I would never advise you to touch the coins you're holding at any cost cos once its tampered, the possibility of it getting finished is high.

 One thing we need to understand is that you must not victimize yourself to fulfill a desire. NO, it calls for more creativity on your path. You should create a money generating avenue to contain That charity you intend doing which is another thing all together and must be treated as such. Therefore, you must hustle differently for that one on your own without touching your 100% holding. Try to find another source of acquiring the money you need for the charity you intend doing. That way, you'll not break your principles, and you'll fulfill your desire of helping the bitcoin community. Then you'll be glad that you've finally won in the two sides.

Why not a strategic, limited transaction allocation? Smart diversification, not abandoning hodling, is the goal. You're not just an investor; you're a participant in the Bitcoin economy.

Investing in Bitcoin requires knowing and engaging in its ecosystem. Using a portion of your holdings to support Bitcoin-accepting businesses increases its real-world utility and adoption. Remember that Bitcoin's value grows with usage and acceptance, not hoarding. You may think you're deviating from your strategy, but arent you strengthening Bitcoin's value?

Of course that's a logical suggestion - capital and investment budgets should be kept separate, otherwise OP would have to come up with a capital budget out of her own pocket. Mixing things up would only mess up his plans - usually it wouldn't last long before separate each money into two parts.

The OP can set aside some of the profits from his business to build his investment portfolio - while the capital and some of the profits should be able to be turned back into building a better business. That's how business is run even if you are a long-term bitcoin holder.

I have come across of a thing of this nature concerning bitcoin been accepted for business, acceptance of bitcoin in a business that established by you or managed by you is good thing and is another kind of way to ensure that you have boasts of your economy, because acceptance of bitcoin for your business, its two things you are doing at same time, the first one is that you have put your money in another way whereby it will get increased at any point of time, and secondly you have secured your money from tradinational money and expenses also, if you want to hold your bitcoin I think its very ideal for you to divert it to your business and people who is interested to with bitcoin can equally make deposit with bitcoin, some businesses currently support a payment with bitcoin.

I feel that it depends on the amount of bitcoin you are holding and how much of it you will be willing to give out in support of this business and it will not affect you in the long run. Understand that this is your own act of benevolence and you shouldn't put yourself in a situation where you will regret your actions will you must have used a reasonable amount of your hildings to support business and suddenly the price of bitcoin hits very high. You need to ask yourself the question regarding your willingness to give out those holding to business and you should also be very sincere with the answers you give back to yourself. Just sit back and count the cost of taking either of the options you outlined and you will get sure answers from within you.

What good in that business accepting BTC for you? Is that your business? If not then it means it belongs to someone else but how big is that business? let's say the business is huge and this will attract more consumers using BTC as a payment method this will increase the utilization of BTC and it is obvious that high utilization means more demand and more price overall.
But, I will say it is better to hold 100% because this business that is accepting BTC as a payment method is not bigger than any of the BTC ETFs that are going to be approved soon. If it's true then the most beneficial decision would be to hold 100% and then you could consider supporting the BTC utilization. I don't even know how much BTC you have that you want to add to the utilization of BTC but if you are aiming to spend some then what's bad in doing fifty-fifty?
This way, you can get some of your holdings with you and can add some to the utilization of BTC also. But before that clarify your purpose and what you want from this.

If you are opening a business accepting Bitcoin, why should it bother you to hold Bitcoin? In fact, you should be able to do both (open your business that accepts Bitcoin and keep holding the Bitcoin you have for long-term investment)
with bitcoin price fluctuations constantly changing, you should be able to take advantage of that and try to make a profit by accepting Bitcoin as payment. but my question is whether your country is friendly for you to do that (open a business that accepts bitcoin), you must pay attention to things related to government regulations, you can't do that alone without safe regulations from the government.