The amount of investment is increasing, and its value is increasing enough, it is higher.
This makes some institutions that invest in Bitcoin have quite a lot of profits.

Let's see, how Tesla was initially quite serious about Bitcoin, Until now Tesla continues to collect Bitcoin.

Total Bitcoins held: 9,720 BTC and Current investment value (April 5, 2024): $324 million.
Tesla has also sold some of their Bitcoin holdings in 2022 by about 75% with the value of Bitcoin at the time $926 Million.
But now they're starting to re-enter and buy gradually, and Elon Musk says it's going to be held for the long term.

Yes those are some of the companies that have invested heavily in bitcoin this year and I also read a flash news about MicroStrategy that the company has acquired more bitcoins of that amount to $2,5 billions but I can't really get the exact figure again and little research on it provide this to me but I will not rely on this for now. https://www.bloomberg.com/news/articles/2024-03-19/microstrategy-owns-about-1-of-all-bitcoin-with-latest-purchase-mstr
But I will still check on fillippone thread to see the exact amount. And there is one company which is competing with microstrategy in possession of bitcoin.

As far as I know, the two largest companies currently investing in Bitcoin and holding the largest amounts of Bitcoin are MicroStrategy and BlackRock.

Of course, there are many other companies, but these two companies are the largest in terms of the number of Bitcoins they own, and they are constantly working to increase their possession of Bitcoins.

Of course, then come the ETF companies in general, which are also trying to increase their stocks. Distributing Bitcoin among this company is better for the market and decentralization.

Not only institutions, we should think about the countries who are investing in bitcoin too, like El Salvador, they made public their cold wallets some weeks ago, and they are holding more than 5,000 BTC now.

You can see on the next link the companies who have invested in Bitcoin and how many BTC they have, the list was posted on 9/Jan/2024
https://www.statista.com/statistics/1228305/publicly-listed-companies-with-bitcoin/

MicroStrategy (U.S.)   189,150
Marathon Digital Holdings Inc (U.S.)   15,174
Tesla, Inc (U.S.)   10,725
Hut 8 Corp (Canada)   9,195
Coinbase Global, Inc. (U.S.)   9,000
Galaxy Digital Holdings (U.S.)   8,100
Block, Inc. (U.S.)   8,027
Riot Platforms, Inc. (U.S.)   7,362
Bitcoin Group SE (Germany)   3,830
CleanSpark Inc (U.S.)   3,002
Voyager Digital LTD (Canada)   2,287
NEXON Co. Ltd (Japan)   1,717
HIVE Digital Technologies (Canada)   1,707

   
1.Barry Silbert is the founder of Digital Currency Group, which invests in popular cryptocurrency and blockchain companies.
Digital Currency Group has invested in 218 blockchain- and cryptocurrency-related companies.Other portfolio companies include Genesis, a liquidity provider—an entity that funds a pool with cryptocurrency—for cryptocurrency buyers and sellers in the over-the-counter market. Digital Currency Group also invests in Grayscale Investments, which manages the Bitcoin Trust (GBTC), an investment vehicle that holds $15.6 billion in bitcoin as of Feb.19, 2023, which gives investors exposure to its price movements without requiring them to hold any directly.
2. Michael Saylor is the CEO of MicroStrategy, which is investing heavily in Bitcoin.
The co-founder and CEO of business intelligence firm MicroStrategy, Michael Saylor, believes that cryptocurrency is the next biggest investment. MicroStrategy increased its bitcoin holdings in 2022 by purchasing 8,813 bitcoins at an average price of $32,670. The company holds a total of 132,500 bitcoins as of Feb. 19, 2023.


3.Cameron and Tyler Winklevoss are believed to be the first Bitcoin billionaires, reportedly holding about 70,000 coins.
Cameron and Tyler Winklevoss are investors in several cryptocurrencies and blockchain-related businesses, most notably the privately-owned Gemini exchange. They are believed to be the first to reach billionaire status by investing in Bitcoin, reportedly holding about 70,000 coins, according to Forbes.4

The Winklevoss twins launched the Gemini exchange in 2015.7 The company allows investors to buy, sell, and store their digital assets. Gemini launched bitcoin futures on the CBOE Options Exchange (CBOE) in December 2017.8



4.Elon Musk's company Tesla purchased $1.5 billion in Bitcoin in 2021 but is down to $191 million as of its 2022 annual filings.Elon Musk is the CEO and co-founder of Tesla (and other companies). Tesla engineers and builds electric vehicles and clean energy products. As an innovative company standing at the forefront of technology, it should be expected that it would own cryptocurrency—Tesla purchased $1.5 billion worth of bitcoin in 2021 to begin accepting it as a form of payment.9 As of its 2022 annual report, the company has converted 75% of its digital assets, reducing its holdings to $191 million.
5. Michael Novogratz founded Galaxy Digital Holdings, a broker-dealer and cryptocurrency and digital asset management firm.

Galaxy Digital focuses on digital assets; in the third quarter of 2021, it purchased $62 million worth of non-fungible token (NFT) related companies.10 NFTs are digital assets that use blockchain technology for security and ownership identification. They became popular in 2021 after digital art secured by NFT technology began selling for millions of dollars.

Galaxy reported about $2 billion in assets under management in its third-quarter 2022 results, with $875 million of those assets used in its fund products.

Source:https://www.investopedia.com/articles/people/091516/top-5-investors-investing-bitcoin.asp

Wow, the 2nd in the leaderboard is so far from the 1st place, that's a really good way to see how dominant MicroStrategy is when it comes to bitcoin accumulation, there's got to be a threshold as to when they're going to sell any amount of that bitcoin, definitely not a bad idea if they do right now and it's going to be chaotic for the market if they ever sell because it's definitely going to make the price go down once MS takes profit and a lot of these companies in the leaderboard will definitely follow MS and take their own profits, we're going to see bitcoin go down more than we've ever seen, that's a guarantee and with that possibility it's probably a good idea to start saving some fiat now and be ready for the case that you're going to need to buy because of how low the price of bitcoin is.

Except for Microstrategy, all these other companies are real dwarfs with their investments considering how much they are actually worth. It seems to me that some of these companies are buying Bitcoin just to draw a little more attention to themselves, but they are far from serious investors.

As for Tesla and their great leader, some information appeared that they started buying BTC again, although some say that it might just be an accounting error.

1. Large financial institutions such as JPMorgan Chase, Goldman Sachs, and Citibank.
2. Hedge funds like Renaissance Technologies and Bridgewater Associates.
3. Technology companies such as Square and MicroStrategy.
4. Sovereign wealth funds from countries like Singapore and Norway.

In the short term, this is positive, but I fear these massive players are nothing more than an attempt at control and mass accumulation. What I fear the most is what will happen when the supply dries up, and the only people holding are these traditional industries—everything that Bitcoin should be going against.

Well, there are many of them to be honest, and personally, I won't be able to name them all, but first, we much recognize Microstrategy for that company's strong and unwavering believe in the the future of bitcoin and cryptocurrencies in general.

And after bitcoin Spot ETF was approved, major asset holders like Blackrock, Grayscale, Vanguard and so on and on all purchased various numbers of bitcoin, and even after the ETF was approved, I trust that this institutions plus alot of others have also purchased a lot of bitcoins as well.

Tesla is also a very popular name in the world of bitcoin investment, Elon musk announced when tesla bought its bitcoins, but later on, also announced when tesla also sold some of the bitcoin, and till date, Elon musk have never made any annoument again on Tesla selling it's remaining part of bitcoin they bought, which suggests that the company is still holding the remaining part of their bitcoin stash they kept after selling some.
Elon musk himself, I believe owns alot of bitcoins.

And speaking on the effect this has, or will have on the price of bitcoin, what other effect will it have if not that this will make the price of bitcoin go up significantly in the future, all this names are really big names and are likely not to sell their bitcoins anytime soon, so, while all this bitcoin are held in wallets that may never move them for several years from now, there are less bitcoin in the market and the more demand for bitcoin increases, so will the price of bitcoin as well will increase.